Biotin-PEG-NHS (biotin-polyethylene glycol-active ester)—focus on PEG derivatives

Biotin-PEG-NHS (biotin-polyethylene glycol-active ester) is a type of biotin PEG derivative. Biotin and streptavidin have good affinity. The biotin-avidin system is currently widely used in trace antigen, antibody qualitative and quantitative detection and diagnosis (ELISA). Biotin is easy to bind to biological macromolecules such as proteins and nucleic acids. The formed biotin derivatives not only maintain the original biological activity of macromolecules, but also have high specific gravity and multivalency.

In addition, each avidin molecule has four biotin binding sites, which can simultaneously bind biotinylated macromolecular derivatives and markers in a multivalent form. Active ester (NHS) PH7-8.5 reacts with primary amine groups to form stable amide bonds. Through PEG connection, biotin can become a derivative with multiple active groups-activated biotin. Activated biotin can be coupled with almost all known biological macromolecules, including proteins, nucleic acids, polysaccharides, lipids, etc., under the mediation of protein crosslinkers.

Biotin PEG active ester (BIOTIN-PEG-NHS) can be used to modify proteins, peptides and other materials or small molecules. NHS reacts with primary amine groups at pH 7-8.5 to form stable amide bonds.

Biotin functionalized polyethylene glycol (PEG-Biotin) is a PEG derivative conjugated to avidin or streptavidin, used to biotinylate the surface of biomolecules or other materials. Biotin can be easily detected by biotin/streptavidin binding assay and is widely used for molecular target detection.

English name: Biotin-PEG-NHS

English alias:

Biotin-PEG-SC

NHS-PEG-Biotin

SC-PEG-Biotin

Molecular weight: 200, 400, 500, 600, 800, 1k, 2k, 3.4k, 5k, 6k, 8k, 10k, 20k (customizable)

Product purity:

≥95%

Product use:

Applied in medical research, drug release, nanotechnology and new materials research, cell culture. And ligand research, peptide synthesis support, grafted polymer compounds, new materials and polyethylene glycol modified functional coatings and other active compounds.